Journal: Frontiers in Physiology
Article Title: Inositol-requiring enzyme 1 alpha is essential for dentinogenesis
doi: 10.3389/fphys.2025.1722417
Figure Lengend Snippet: Immunohistochemistry staining of phosphorylated IRE1α (pIRE1α) protein in the mandibular first molars. (A) Shown are the representative images of immunohistochemistry staining of pIRE1α protein (signal in brown) in the mandibular first molars of 3-week-old Dspp +/+ , Dspp P19L/+ , and Dspp P19L/P19L mice. Each image in (A) is from the middle region of the crown of a sagittally-sectioned mandibular first molar. (A1-A3) are the higher magnification views of the roof-forming odontoblasts (box1), dental pulp cells (box 2) and floor-forming odontoblasts (box 3) in the corresponding images in (A) , respectively. rd, roof dentin; fd, floor dentin; rod, roof-forming odontoblasts; fod, floor-forming odontoblasts; Scale bars: 50 μm in A; 20 μm in (A1-A3). Three independent experiments for IHC staining of pIRE1α show similar results.
Article Snippet: The primary antibodies used in this study included: 1) rabbit anti-phosphorylated IRE1α polyclonal antibody (1:400, Novus Biologicals, NB100-2323); 2) rabbit anti-XBP1 polyclonal antibody that recognizes both unspliced XBP1 (XBP1U) and spliced XBP1 (XBP1S) (1:200, Abcam, ab37152); 3) rabbit anti-XBP1S monoclonal antibody (E9V3E) that specifically recognizes XBP1S ( ; ) (1:50, Cell Signaling Technology, Danvers, MA); 4) rabbit anti-DSPP polyclonal antibody that recognizes both DSP and full-length DSPP (1:2000) ( ); and 5) rabbit anti-DMP1 polyclonal antibody (1:800, #857) ( ).
Techniques: Immunohistochemistry, Staining